The New Detection Paradigm
The PAIA approach uses the well-known advantages of bead-based immunoassays with fluorescence detection in a much simplified workflow.
We have reinvented bead-based assays with a new detection principle that leads to streamlined assay protocols. We measure the fluorescence marker in solution rather than quantifying the fluorescence that has bound to the beads, which is common to other technologies. This requires the washing of the beads and therefore leads to more complicated and error-prone protocols.
Washing steps can be omitted in the PAIA workflow thanks to the PAIAplates. These special microplates allow efficient incubation of the capture beads, analyte and marker (B) and separation of capture beads from the solution (A) in such a way that they do not interfere with the measurement of fluorescence marker in solution.
PAIA assays employ capture beads that bind the analyte molecule, and uses a fluorescence marker to detect the binding of the analyte to the beads. This can be achieved in two ways: in direct assay formats the analyte enables the binding of the marker to the beads and in competitive assay formats the analytes releases the marker from the beads, leading to different assay response curves for the two assay formats.